Method for acquiring information of nano antibody for specifically recognizing antigen

• Main Authors: LIU YA, WANG MEINIANG, SUN NA, LIU YANG, LIANG YUANQING, LYU MENGHUA, LIU LONGQI, LIU XIAOPAN, REN BINGZHAO, GAO KAI, WANG SHIYU, SHEN MENGZHE, ZHENG YUE
• Format: Patent
• Language: Chinese; English
• Published: 27.08.2024
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

The invention provides a method for acquiring information of a nano antibody for specifically recognizing an antigen. The method comprises the following steps: enriching B cells from peripheral blood lymphocytes of a subject; the method comprises the following steps of: wrapping a solution containing an antigen and a signal detection reagent and a single B cell in a single liquid drop by utilizing a microfluidic liquid drop generation chip; incubating the generated single liquid drop, so that the B cell secretes a nano antibody, and the antigen is specifically combined with the nano antibody; sorting the incubated liquid drops by using a liquid drop sorting chip, analyzing the collected detectable signal so as to sort target cells capable of secreting the target nano antibody, and collecting positive liquid drops containing the target cells; and carrying out single cell sequencing on the target cells so as to obtain target nano antibody information. 本发明提出了获取特异性识别抗原的纳米抗体信息的方法，包括：从受试者的外周血淋巴细胞中富集B细胞；利用微流控液滴生成芯片，