Indel marker CmaWSC linked with color of seed coat of pumpkin as well as primer, kit and method of Indel marker CmaWSC

• Main Authors: WANG CHANGLIN, DUAN YING, BO KAILIANG, SHI YUZI
• Format: Patent
• Language: Chinese; English
• Published: 23.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to an Indel marker CmaWSC linked with a pumpkin seed coat color, and a primer, a kit and a method thereof, and belongs to the technical field of molecular markers and assisted breeding. According to the Indel marker CmaWSC linked with the seed coat color of Cucurbita maxima D., compared with a linkage fragment corresponding to a white seed coat character, a linkage fragment corresponding to a yellow seed coat character has base insertion mutation at a 58th site of the linkage fragment corresponding to the white seed coat character, and a linkage fragment corresponding to a yellow seed coat character has base insertion mutation at a 58th site of the linkage fragment corresponding to the white seed coat character; the base sequence of the insertion mutation is as shown in SEQ ID NO. 5. According to the invention, Indel markers developed by using a white seed coat material Agol and a yellow seed coat material Wumingv of an Indian pumpkin are verified by using 17 varieties, and the result sh