Method for improving transcription factor activation activity through tandem transcription factor activation structural domain

• Main Authors: NING GUOGUI, SHEN YUXIAO, LI RUNHUI, HE CHAOCHAO, CHEN RUNZHOU
• Format: Patent
• Language: Chinese; English
• Published: 23.08.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for improving transcription factor activation activity by serially connecting transcription factor activation structural domains, which comprises the following steps of: performing PCR (Polymerase Chain Reaction) amplification on one or more sequences by taking a nucleotide sequence corresponding to a transcription factor protein sequence as a template; designing a CDS sequence corresponding to a primer amplification transcription factor protein sequence; a transcription factor CDS sequence and an ADn sequence in the transcription factor CDS sequence are recombined to an intermediate vector through homologous recombination to synthesize the artificial transcription factor. According to the present invention, the synthesized series of the artificial transcription factors of the tandem transcription factor activation structural domain have strong transcription activation ability, the new strategy and the new method are provided for the transformation of the transcription factor