Heat-resistant cellulose endonuclease Cel12E mutant with improved alkali resistance and application of heat-resistant cellulose endonuclease Cel12E mutant

The invention relates to a heat-resistant cellulose endonuclease Cel12E mutant with improved alkali resistance and application of the heat-resistant cellulose endonuclease Cel12E mutant. According to the invention, heat-resistant cellulase Cel12E from hyperthermophilic archaea in deep sea of a GH12...

Full description

Saved in:
Bibliographic Details
Main Authors YANG FAN, YU ZHIMIN, XU SUMIN
Format Patent
LanguageChinese
English
Published 23.08.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention relates to a heat-resistant cellulose endonuclease Cel12E mutant with improved alkali resistance and application of the heat-resistant cellulose endonuclease Cel12E mutant. According to the invention, heat-resistant cellulase Cel12E from hyperthermophilic archaea in deep sea of a GH12 family is taken as a female parent, and amino acid sites of 65th site, 87th site, 109I site, 126 site, 162 site, 200 site, 216 site, 250 site and 273 site of the heat-resistant cellulase Cel12E are respectively mutated to obtain Cel12E (Q65A), Cel12E (D87H), Cel12E (P109I), Cel12E (S126V), Cel12E (T162Y), Cel12E (R200I), Cel12E (S216V), Cel12E (Q250I), Cel12E (T273 V) and Cel12E ( The ten mutant enzymes comprise Cel12E (S126V), Cel12E (R200I), Cel12E (S216V), Cel12E (T273 V), and Cel12E (R200Iamp; T273), and the ten mutant enzymes comprise Cel12E (S126V), Cel12E (R200I), Cel12E (S216V), Cel12E (T273 V) and Cel12E (R200Iamp; t273V), which is a mutant of which the enzyme activity is remarkably improved under an alkal
Bibliography:Application Number: CN202410792159