Cytosine base editing system and application thereof

• Main Authors: TANG CHENGCHENG, LU XUAN, CHEN MIN, SUKEKANE, ZHOU XIAOQING, NING LI, ZOU QINGJIAN, LIN ZIYI, GAO YUE, XI JIAHUI
• Format: Patent
• Language: Chinese; English
• Published: 06.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a cytosine base editing system and application thereof, and particularly discloses a fusion protein which sequentially comprises a first fusion protein and a second fusion protein from an N terminal to a C terminal, the first fusion protein sequentially comprises an FK506 binding protein fragment, a deaminase first fragment, an nCas9 first fragment and two uracil glycosyl enzyme inhibition protein fragments from the N end to the C end; the second fusion protein sequentially comprises an nCas9 second fragment, a deaminase second fragment and an FKBP rapamycin binding structural domain from the N end to the C end. The fusion protein provided by the invention can respond to dimerization of rapamycin so as to exert gene editing activity, and the fusion protein is low in editing off-target rate and high in editing efficiency. 本发明公开了一种胞嘧啶碱基编辑系统及其应用，具体公开了一种融合蛋白，所述融合蛋白自N端至C端依次包括第一融合蛋白和第二融合蛋白；所述第一融合蛋白自N端至C端依次包括FK506结合蛋白片段、脱氨酶第一片段、nCas9第一片段和两个尿嘧啶糖基酶抑制蛋白片段；所述第二融合蛋白自N端至C端依次包括nCas9第二片段、脱氨酶第二片段和FKBP