Nucleic acid enrichment detection method based on condensed liquid drops

• Main Authors: QI CHENG, MA XUDONG, LIU ZHOU, CEN CHAOFENG, KONG TIANTIAN
• Format: Patent
• Language: Chinese; English
• Published: 02.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the field of biological materials, and particularly relates to a nucleic acid enrichment detection method based on condensed liquid drops. The invention provides a method for separating and enriching and concentrating biomolecules based on condensed liquid drops. The reaction process of the biomolecules is promoted. The process of preparing the condensed liquid drops is simple, automatic separation and enrichment of biomolecules are achieved, and complex pretreatment steps are not needed. According to the method, two polymer aqueous solutions are mixed, the condensed liquid drops are directly generated in an oscillating or shaking mode, the method is simple and rapid, complex instruments are not needed, and the enrichment efficiency is high. 本申请属于生物材料领域，具体涉及一种基于凝聚液滴的核酸富集检测方法。本申请提供了一种基于凝聚液滴的生物分子分离和富集浓缩的方法，促进了生物分子的反应过程。制备的凝聚液滴过程简单，实现对生物分子的自动分离富集，而无需复杂的预处理步骤。通过使用两种聚合物水溶液之间的混合，采用震荡或者摇晃的方式直接生成凝聚液滴，方法简单快捷，无需复杂的仪器，还具有较高的富集效率。