Enrichment culture method and application of acetochlor degrading strain

• Main Authors: XU MINGKAI, GUAN XINYU, SU ZHENCHENG, LI XU, ZHANG HUIWEN, DAI YUMENG, LI XIANG, WANG XIUJUAN
• Format: Patent
• Language: Chinese; English
• Published: 26.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINATED SOIL; RECLAMATION OF CONTAMINED SOIL SOIL; SPIRITS; TRANSPORTING; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; VINEGAR; WINE

The invention discloses an enrichment culture method and application of an acetochlor degrading strain. The enrichment culture method comprises the following steps: carrying out enrichment culture on a material containing the substrate in an enrichment culture medium; the enrichment culture medium comprises sodium salt, trace elements, sylvite, ammonium salt, a calcium-magnesium solution and vitamins; the material is soil or activated sludge. According to the present invention, by using the enrichment culture, the acetochlor can be substantially reduced by using the acetochlor degrading bacterial system, the content of the sphingomonas in the bacterial system is high, and the acetochlor degrading rate and the acetochlor degrading stability of the bacterial system are significantly improved compared with the existing enrichment culture mode. 本发明公开了一种乙草胺降解菌系的富集培养方法及应用。所述富集培养方法包括将含所述底物的材料在富集培养基中进行富集培养；所述富集培养基包括钠盐、微量元素、钾盐、铵盐、钙镁溶液和维生素；所述材料为土壤或活性污泥。通过使用富集培养，乙草胺降解菌系能够大幅消减乙草胺，在该菌系中，鞘脂单胞菌属(Sphingomonas)占比较高，较现有的富集培养方式