Recombinant bacterium for producing D-lactic acid through co-utilization of glucose and xylose as well as construction method and application of recombinant bacterium

• Main Authors: ZHU XINNA, QIAO JIALE, FANG YU, ZHANG XUELI, XI YONGYAN, LIU RU, ZHANG YIWEN
• Format: Patent
• Language: Chinese; English
• Published: 23.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a recombinant bacterium for producing D-lactic acid through co-utilization of glucose and xylose as well as a construction method and application thereof. The invention relates to the technical field of biology, in particular to recombinant bacteria for producing D-lactic acid through co-utilization of glucose and xylose as well as a construction method and application. Methylglyoxal synthase, and/or pyruvate formate lyase, and/or fumarate reductase and/or acetaldehyde/ethanol dehydrogenase in receptor escherichia coli Rec1.0 are modified, recombinant escherichia coli obtained through evolution and domestication is fermented for 96 h, lactic acid is the only metabolite, and the yield of lactic acid reaches 87.5 g/L; the consumption rate of xylose is 0.82 g/gDCW.h after fermentation for 24 h, and the consumption rate of glucose is 0.75 g/gDCW.h. The recombinant Escherichia coli constructed by the invention can realize efficient utilization of lignocellulose hydrolysate (containing gluco