Chemiluminescence immunodetection kit and application thereof in immunodetection

• Main Authors: TANG SHIYU, WU HAOQI, FAN GUILIANG, CAO HAO, DUAN XUEJUN, NI HAI'OU
• Format: Patent
• Language: Chinese; English
• Published: 16.07.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of in-vitro detection, and particularly relates to a chemiluminescence immunodetection kit and application thereof in immunodetection. According to the chemiluminescence immunodetection kit provided by the invention, the first target protein recognition molecule and the second target protein recognition molecule capture proteins respectively, and horse radish peroxidase on the second target protein recognition molecule is subjected to polymerization reaction with the chemiluminescence reagent and noradrenaline to form a product enriched with the chemiluminescence reagent; a chemiluminescence signal is directly generated under the action of an alkaline pH regulator and hydrogen peroxide, and protein recognition molecules do not need to be covalently modified by a chemiluminescence reagent, so that the loss of biomacromolecule activity is reduced, the utilization rate of the protein recognition molecules is increased, the batch difference of the luminescence reagent