Method for identifying adulterated spina date seeds in spina date seeds

• Main Authors: WANG RONG, ZHOU YING, LU JINGXIAN, CHEN BILIAN, ZHENG CHENG, YORISAWA
• Format: Patent
• Language: Chinese; English
• Published: 16.07.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

According to the method, O-nornuciferine is adopted as a detection index, after sample powder is extracted, ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry is adopted for analysis, and an electrospray ionization source positive ion mode and a multi-reaction monitoring mode are adopted for identifying the adulterated spina date seeds in spina date seeds. The mass-to-charge ratio (m/z) of 281.9 to 251.1 and/or 281.9 to 219.1 is selected as a detection ion pair; the content of O-nornuciferine is calculated according to the peak area, so that whether jujube kernels are adulterated in the sample or not is judged. The method disclosed by the invention has the advantages of simple steps, convenience in sample pretreatment, high sensitivity and good stability, and solves the technical problem that the adulterated spina date seeds in the spina date seeds are difficult to accurately identify; different from conventional form and appearance identification, the method adopts a trip