Elastase and preparation method thereof

The invention discloses elastase and a preparation method thereof. The preparation method comprises the following steps: (1) extracting; (2) ultrafiltration and concentration: controlling the temperature of the cooled filtrate obtained in the step (1) to 5-10 DEG C, filtering with filter paper, perf...

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Main Authors TANG ZHANGYONG, LI XIANFENG, ZHOU SIYU, LI YUAN, ZHANG ZHONGYUAN, ZHOU HOUJIE, WU MENGXIA, HWANG JUNG-KYUN, XIAO YONG, ZHANG GE, WANG QIAN
Format Patent
LanguageChinese
English
Published 09.07.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses elastase and a preparation method thereof. The preparation method comprises the following steps: (1) extracting; (2) ultrafiltration and concentration: controlling the temperature of the cooled filtrate obtained in the step (1) to 5-10 DEG C, filtering with filter paper, performing ultrafiltration and concentration with a hollow fiber membrane, and dialyzing with purified water at 5-10 DEG C until the conductivity is less than or equal to 5.0 ms to obtain ultrafiltrate; (3) column chromatography separation: adjusting the pH value of the ultrafiltrate in the step (3) to 5.5-5.6, filtering and clarifying, controlling the temperature of the clear liquid to be less than or equal to 10 DEG C, enabling the filtrate to pass through an LXPC-958 anion resin chromatographic column, collecting a flow-through liquid and a flushing liquid, enabling the flushing liquid to continuously pass through DEAE ion exchange resin, and collecting the flow-through liquid and the flushing liquid; (4) flow-throu
Bibliography:Application Number: CN202410513156