Method for evaluating killing effect of NK (Natural Killer) cells on ovarian cancer organs in vitro

• Main Authors: CHENG YINAN, LIU XIAODONG, HE ZHIYONG
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a method for evaluating the killing effect of NK cells on ovarian cancer organs in vitro. The method comprises the following steps: (a) providing a solid matrigel layer; (b) adding the ovarian cancer organoid, and standing for at least 12 hours; (c) adding NK cells, and co-culturing for at least 12 hours; and (d) detecting the cytotoxicity of the NK cells on the ovarian cancer organs. The established in-vitro evaluation system for the killing effect of the ovarian cancer organoid is suitable for NK cells from various sources, experiments prove that the result of the in-vitro evaluation system is basically consistent with the treatment effect of a clinical corresponding case, and the in-vitro evaluation system truly simulates the in-vivo environment and has important significance on prediction of the killing effect of the NK cells. 本发明提供了一种体外评价NK细胞对卵巢癌类器官杀伤效果的方法，包括：(a)提供固体基质胶层；(b)加入卵巢癌类器官，静置至少12小时；(c)添加NK细胞，共培养至少12小时；(d)检测NK细胞对卵巢癌类器官的细胞毒性。本发明建立的卵巢癌类器官杀伤效果的体外评价体系适用多种来源的NK细胞，通过实验证实，体外评价体系的