Method for synthesizing ceramide precursor through enzyme catalysis

• Main Authors: YU HUILI, DENG DI, FANG LIN, CHEN JULIN, LI AITAO
• Format: Patent
• Language: Chinese; English
• Published: 02.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for synthesizing a ceramide precursor through enzyme catalysis, the ceramide precursor is alpha-hydroxystearic acid, and the method specifically comprises the following steps: by taking stearic acid as a substrate, under the condition of sodium ascorbate, catalyzing alpha-hydroxylation reaction through cytochrome P450 monooxygenase to obtain the ceramide precursor. Compared with chemical synthesis, the method obviously shortens the synthesis route of the alpha-hydroxystearic acid, has the characteristics of low cost, mild reaction conditions, high selectivity, simplicity in operation, safety, high efficiency, high yield and the like, and provides an ideal synthesis route for green and efficient synthesis of the alpha-hydroxystearic acid. 本发明公开了一种酶催化合成神经酰胺前体的方法，其中神经酰胺前体为α-羟基硬脂酸，该方法具体为：以硬脂酸为底物，在维生素C钠条件下，通过细胞色素P450单加氧酶催化α-羟基化反应即得。相对于化学合成，本发明方法明显缩短了α-羟基硬脂酸的合成路线，而且具有成本低廉、反应条件温和、选择性高、操作简单，安全高效和产量高等特点，为绿色高效合成α-羟基硬脂酸提供了一条理想的合成路线。