Anti-zearalenone nano antibody and preparation method thereof

• Main Authors: ZHU WEI, ZHANG ZHILIN, LIN DANRONG, CHEN GANYING, WANG RUITING, FENG QINGQING, LIAO JIANMING, YANG CHUNYUAN, HUANG MIAN, LIAO JUNBIN, ZENG YING, CHEN JINXIAN
• Format: Patent
• Language: Chinese; English
• Published: 02.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an anti-zearalenone nano antibody and a preparation method thereof, and belongs to the technical field of immunity. Specifically, the invention discloses a method for preparing the anti-zearalenone nano antibody, and also discloses an amino acid sequence for coding the nano antibody, the sequence is shown as SEQ ID NO.1, and the sequence comprises four frame regions FR1, FR2, FR3 and FR4 and three complementary determining regions CDR1, CDR2 and CDR3. The method comprises the following steps: immunizing alpaca by adopting a ZEN-BSA complete antigen, extracting peripheral blood lymphocyte RNA, amplifying to obtain a VHH gene, and constructing a phage display nano antibody library; the positive bacteriophage clone is identified through four rounds of affinity screening and a phage-ELISA method; and transforming the cloned plasmid obtained by screening into non-inhibitory escherichia coli for induced expression, purifying and identifying the nano antibody. The molecular weight of the prep