Novel monoclonal antibodies against L-thyroxine and diagnostic uses thereof

• Main Authors: PRENCIPE, GREGORY, GERG MICHAEL, HOLDOTSON, STEPHEN, HEUER CHRISTOPHER D, LEHMANN HENRY, MACINA, ANTONIO, VON PROFF LEOPOLD, JOSEL HANS-PETER, JURKNISCHKE, UWE, SCHIESEL KATRIN, SCHREMM MICHAEL
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2024
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

The present invention provides novel monoclonal antibodies that specifically bind to L-thyroxine (T4), as well as compositions and kits comprising such antibodies. In addition, polynucleotides encoding such monoclonal antibodies, host cells expressing the antibodies, methods of producing such antibodies, and diagnostic methods of using such monoclonal antibodies are provided. The monoclonal antibody of the invention comprises: a heavy chain variable domain (VH) comprising V or A at position 33; y at position 50; w at position 52; i at position 98; g, A or V at position 99; y at position 100; and I at position 100b; and a light chain variable domain (VL) comprising an amino acid H or Y at position 28; n or K at position 29; w at position 32; g or A at position 91; y, W or F at position 92; s or T at position 93; y or F at position 95b; n, S, T or Q at position 95c; and H at position 96, wherein the positions of amino acids in the VH and the VL are respectively indicated according to the Kabat numbering scheme.