Low-temperature culture method of CHO (Chinese hamster ovary) cells for expressing dulatide

• Main Authors: DENG QUANYU, GUO WEI, ZHOU RUOFEI, WANG XIABIN
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a low-temperature culture method of CHO (Chinese hamster ovary) cells for expressing dulatide, which is a low-temperature culture method for prolonging culture time and maintaining high cell viability, and is characterized in that CHO K1SV (Chinese hamster ovary) cells are used as culture objects, and optimization experiment research is carried out on two factors, namely cooling temperature and cooling time; the first-stage cooling time is determined to be the third day of culture and the temperature is reduced to 35 DEG C for culture, the second-stage cooling time is determined to be the sixth day of culture and the temperature is reduced to 30 DEG C for culture, and the second-stage cooling temperature is maintained until culture is finished. The method can prolong the culture time, improve the expression quantity of supernatant, maintain the high cell viability, reduce the proportion of acid peaks and improve the product quality. 本发明公开了一种用于表达度拉糖肽的CHO细胞的低温培养方法，其是一种延长培养时间，维持细胞高活率的低温培养