Enzymatic synthesis process of 5-methyltetrahydrofolic acid

• Main Authors: MA MUQING, ZHANG XUEQI, DONG LEICHI, QU MAOHUA, LUO ZHIBO, LU XIAFENG, WU YAN, LIU SHEN
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses an enzymatic synthesis process of 5-methyltetrahydrofolic acid, the yield is improved by shortening a 5-MTHF (Methyl Tetrahydrofolic Acid) synthesis route in a biological fermentation method, and meanwhile, a general folic acid raw material in a chemical synthesis method is adopted as a starting substrate, so that the production cost of the 5-MTHF is greatly reduced; and the method has the related advantages of a biological method, such as low pollution and simplicity and convenience in operation. Compared with the existing chemical synthesis method and biological fermentation method, the method has great advantages in the aspects of yield and production cost. 本发明公开了一种5-甲基四氢叶酸的酶法合成工艺，通过缩短生物发酵法中5-MTHF合成路线来实现产率的提高，同时采用化学合成法通用的叶酸原料作为出发底物，大大降低了5-MTHF的生产成本，并且兼具生物法的相关优势比如低污染、操作简便等特点。相比于目前的化学合成法和生物发酵法，本发明在产率和生产成本方面具有极大优势。