Method for improving cell resuscitation motility rate

• Main Authors: CHEN XIAOYANG, DENG QUANYU, ZHOU RUOFEI
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for improving cell resuscitation viability, which comprises the following steps: by taking CHO cells as research objects, respectively carrying out gradient optimization experiment research on five factors, namely 37 DEG C water bath time, culture medium temperature, centrifugal temperature, centrifugal force and centrifugal time, and determining that water bath is carried out for 2 minutes at 37 DEG C, culture medium washing is carried out at 4 DEG C, culture medium resuspension is carried out at 37 DEG C, centrifugal force is 200g, and centrifugation is carried out for 5 minutes at 4 DEG C; after incubation for half an hour, the viability of the recovered CHO cells can be remarkably improved. 本发明公开了一种提高细胞复苏活率的方法，该方法以CHO细胞为研究对象，分别对37℃水浴时间、培养基温度、离心温度、离心力和离心时间五个因素开展梯度优化实验研究，确定了37℃水浴2分钟，4℃培养基洗涤和37℃培养基重悬，离心力为200g、4℃离心5分钟，孵育半个小时，可以显著提升复苏的CHO细胞的活率。