GENETIC CONSTRUCT FOR TRACKING AND/OR ABLATING QUIESCENT CELLS

• Main Authors: TUBRY LUCA, ANTONICA FRANCESCO
• Format: Patent
• Language: Chinese; English
• Published: 21.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A genetic construct is described, the genetic construct comprising: a nucleotide sequence A (Cre + ERT2), the nucleotide sequence A (Cre + ERT2) comprising a nucleotide sequence SEQ ID NO. 1 encoding a recombinase Cre and a sequence SEQ ID NO. 2 encoding a mutant estrogen receptor ERT2; or a nucleotide sequence A'comprising a nucleotide sequence SEQ ID NO.3 encoding a diphtheria toxin fragment A (DTA), and comprising a nucleotide sequence B comprising a nucleotide sequence SEQ ID NO.4 encoding an inhibitor p27K-of mutant cyclin dependent kinase (CDK). 描述了遗传构建体，该遗传构建体包含：核苷酸序列A(Cre+ERT2)，该核苷酸序列A(Cre+ERT2)包含编码重组酶Cre的核苷酸序列SEQ ID NO.1和编码突变型雌激素受体ERT2的序列SEQ ID NO.2；或核苷酸序列A'，该核苷酸序列A'包含编码白喉毒素片段A(DTA)的核苷酸序列SEQ ID NO.3；并且包含核苷酸序列B，该核苷酸序列B包含编码突变型细胞周期蛋白依赖性激酶(CDK)的抑制剂p27K-的核苷酸序列SEQ ID NO.4。