Protease B deletion type hansenula polymorpha strain for expressing HPV 16 L1 protein and application of protease B deletion type hansenula polymorpha strain

• Main Authors: TONG GUANGJIE, YANG WENBIN, LIU ZHILEI, AN YOUCAI
• Format: Patent
• Language: Chinese; English
• Published: 21.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of microorganisms and application thereof, and provides a preparation method of a protease B deletion type hansenula polymorpha strain for expressing HPV (human papillomavirus) 16L1 protein, the protease B deletion type hansenula polymorpha strain for expressing the HPV 16L1 protein, and application of the strain in preparation of the HPV 16L1 protein. The protease B deletion type hansenula polymorpha strain for expressing the HPV 16L1 protein can efficiently express the HPV 16L1 protein, degradation of target protein in the fermentation process is effectively reduced, and the yield of the target protein is increased; and the HPV 16L1 protein obtained by expression has high stability and high purity, can be self-assembled into VLP, is more suitable for commercial production and industrial purification, and has remarkable economic benefits and social benefits. 本发明属于微生物及其应用领域，提供了用于表达HPV 16L1蛋白的蛋白酶B缺失型汉逊酵母菌株的制备方法，用于表达HPV 16L1蛋白的蛋白酶B缺失型汉逊酵母菌株，以及该菌株在制备HPV 16L1蛋白中的应用。本发明提供的用于表达HPV