Cottonrose hibiscus ISSR-PCR reaction system, molecular marking method and application
The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA, 3.5 [mu] l of Easy Taq Buffer (with Mg < 2 + >), 1.5 [mu] l of a primer, 0.2 [mu] l of Taq polymerase and 0.8 [mu] l of dNTPs. And amp...
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Format | Patent |
Language | Chinese English |
Published |
18.06.2024
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Abstract | The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA, 3.5 [mu] l of Easy Taq Buffer (with Mg < 2 + >), 1.5 [mu] l of a primer, 0.2 [mu] l of Taq polymerase and 0.8 [mu] l of dNTPs. And amplifying the reaction system according to the following procedures: pre-denaturation at 95 DEG C for 5 minutes, denaturation at 94 DEG C for 1 minute, annealing of each primer at the optimal annealing temperature for 1 minute, extension at 72 DEG C for 2 minutes, circulation for 40 times, supplementary extension at 72 DEG C for 10 minutes, and preservation at 4 DEG C. The invention also provides an ISSR-PCR molecular marking method for cottonrose hibiscus. A stripe amplified by the established hibiscus mutabilis ISSR-PCR amplification reaction system is high in definition and stability, has very high polymorphism, and makes up for the deficiency of the current research on the genetic diversity of hibiscus mutabilis; the h |
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AbstractList | The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA, 3.5 [mu] l of Easy Taq Buffer (with Mg < 2 + >), 1.5 [mu] l of a primer, 0.2 [mu] l of Taq polymerase and 0.8 [mu] l of dNTPs. And amplifying the reaction system according to the following procedures: pre-denaturation at 95 DEG C for 5 minutes, denaturation at 94 DEG C for 1 minute, annealing of each primer at the optimal annealing temperature for 1 minute, extension at 72 DEG C for 2 minutes, circulation for 40 times, supplementary extension at 72 DEG C for 10 minutes, and preservation at 4 DEG C. The invention also provides an ISSR-PCR molecular marking method for cottonrose hibiscus. A stripe amplified by the established hibiscus mutabilis ISSR-PCR amplification reaction system is high in definition and stability, has very high polymorphism, and makes up for the deficiency of the current research on the genetic diversity of hibiscus mutabilis; the h |
Author | TANG SHENGWEN ZHU ZHANGSHUN MA JIAO ZENG XINMEI LI FANGWEN CHEN XI SHI XIAOQING |
Author_xml | – fullname: LI FANGWEN – fullname: TANG SHENGWEN – fullname: ZENG XINMEI – fullname: ZHU ZHANGSHUN – fullname: SHI XIAOQING – fullname: MA JIAO – fullname: CHEN XI |
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DocumentTitleAlternate | 一种木芙蓉ISSR-PCR反应体系及分子标记方法与应用 |
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Snippet | The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA,... |
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SubjectTerms | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
Title | Cottonrose hibiscus ISSR-PCR reaction system, molecular marking method and application |
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