Cottonrose hibiscus ISSR-PCR reaction system, molecular marking method and application
The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA, 3.5 [mu] l of Easy Taq Buffer (with Mg < 2 + >), 1.5 [mu] l of a primer, 0.2 [mu] l of Taq polymerase and 0.8 [mu] l of dNTPs. And amp...
Saved in:
Main Authors | , , , , , , |
---|---|
Format | Patent |
Language | Chinese English |
Published |
18.06.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The invention relates to a cottonrose hibiscus ISSR-PCR reaction system and application. Every 25 [mu] L of the reaction system contains 50 ng of template DNA, 3.5 [mu] l of Easy Taq Buffer (with Mg < 2 + >), 1.5 [mu] l of a primer, 0.2 [mu] l of Taq polymerase and 0.8 [mu] l of dNTPs. And amplifying the reaction system according to the following procedures: pre-denaturation at 95 DEG C for 5 minutes, denaturation at 94 DEG C for 1 minute, annealing of each primer at the optimal annealing temperature for 1 minute, extension at 72 DEG C for 2 minutes, circulation for 40 times, supplementary extension at 72 DEG C for 10 minutes, and preservation at 4 DEG C. The invention also provides an ISSR-PCR molecular marking method for cottonrose hibiscus. A stripe amplified by the established hibiscus mutabilis ISSR-PCR amplification reaction system is high in definition and stability, has very high polymorphism, and makes up for the deficiency of the current research on the genetic diversity of hibiscus mutabilis; the h |
---|---|
Bibliography: | Application Number: CN202410425844 |