Electrogenerated chemical biosensor for detecting A beta 1-42 protein as well as preparation method and application of electrogenerated chemical biosensor

• Main Authors: CHEN JINGSHUAI, WU MINGYUAN, MAO CHANGJIE, JIANG YUNQI, RYU SUNG-BAE, WEI YUPING
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an electrogenerated chemical biosensor for detecting Abeta1-42 protein as well as a preparation method and application thereof, and relates to the technical field of biosensors, the electrogenerated chemical biosensor is prepared by the following steps: dropwise adding chitosan on a dispersion liquid of CsPbBr3/TDPANCs on the surface of a GCE glassy carbon electrode, incubating an antibody Ab1 of the Abeta1-42 protein on the electrode through a chemical bond, and then adding a pH value regulator, so as to obtain the electrogenerated chemical biosensor for detecting the Abeta1-42 protein. The Ab1 can be subjected to a specific recognition reaction with a target object. According to the prepared electrogenerated chemiluminescence biosensor, energy resonance transfer occurs between CsPbBr3/TDPANCs and Au (at) SiO2NPs, so that an electrogenerated chemiluminescence signal is quenched, and the sandwich-shaped'on-off 'electrogenerated chemiluminescence biosensor is formed. 本发明公开了一种检测Aβ1-42蛋白的