Wide-field photoacoustic microscopic imaging method and wide-field photoacoustic microscopic imaging device

• Main Authors: ZHANG ZHENHUI, YANG SIHUA, SHEN TAO, YANG HONGYA
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2024
• Subjects: CALCULATING; COMPUTING; COUNTING; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASUREMENT OF MECHANICAL VIBRATIONS OR ULTRASONIC, SONIC ORINFRASONIC WAVES; MEASURING; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS; MEASURING LENGTH, THICKNESS OR SIMILAR LINEARDIMENSIONS; PHYSICS; TESTING

The invention discloses a wide-field photoacoustic microscopic imaging method and a wide-field photoacoustic microscopic imaging device, and the method comprises the following steps: a sample is excited by pulsed light to generate a wide-field photoacoustic wave, and the photoacoustic wave causes each mass point on the surface of the sample to generate vibration displacement; detecting instantaneous vibration displacement of each mass point on the surface of the sample under the action of photoacoustic waves at a certain moment by utilizing wide-field interference light; detecting all vibration displacement of each mass point on the surface of the sample under the action of the full-period photoacoustic wave by setting the trigger delay time of the exciting light and the probe light; and reconstructing a wide-field photoacoustic image by performing maximum projection on all the vibration displacements of the mass points on the surface of the sample. The wide-field photoacoustic microscopic imaging device comp