SSR (Simple Sequence Repeat) core primer combination and kit for identifying tea tree variety 'Yinghong No.9' and application of SSR core primer combination and kit

• Main Authors: LI HONGJIAN, QIN DANDAN, NI ERDONG, JIANG XIAOHUI, WANG QING, WANG QIUSHUANG, PAN CHENDONG, WU HUALING, FANG KAIXING, LI BO
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SSR (Simple Sequence Repeat) core primer combination for identifying a tea tree variety 'Yinghong No.9', a kit and application. According to the invention, three pairs of SSR core primers are screened by utilizing an SSR fluorescence labeling detection technology, and the three pairs of SSR core primers can be used for rapidly identifying and detecting the Yinghong-9 from other tea tree varieties. The SSR core primer combination disclosed by the invention can be used for identifying the variety of Yinghong-9, overcomes the uncertainty of identification based on external morphological characteristics, is simple and convenient to operate, high in detection efficiency and reliable and visual in result, and is also beneficial to popularization, utilization and protection of the variety of Yinghong-9. 本发明公开了一种用于鉴定茶树品种"英红九号"的SSR核心引物组合、试剂盒和应用。本发明利用SSR荧光标记检测技术，筛选得到3对SSR核心引物，这3对SSR核心引物可以将"英红九号"从其他茶树品种中快速鉴定和检测出来。本发明所公开的SSR核心引物组合能够对"英红九号"进行品种鉴定，克服了基于外部形态特征鉴定的不确定性，操作简便，检测效率高，结果可靠直观，同时也有利于"英红九号"