Engineering bacterium for strengthening sulfur oxidation function as well as construction method and application of engineering bacterium

• Main Authors: ZHANG WANYU, WANG YUERAN, XIA HAO, YANG MAOHUA, XING JIANMIN, CAO ZIJIA, LYU YONGJIAN, LI HE, SONG BIN
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an engineering bacterium for strengthening a sulfur oxidation function as well as a construction method and application of the engineering bacterium. The construction method comprises the following steps: heterologously expressing sulfur quinone oxidoreductase Sqr in sulfur alkali vibrio cells, and knocking out polysulfide transmembrane transporter protein YilD and sulfur oxidase SoxB to obtain the engineering bacterium for strengthening the sulfur oxidation function. Wherein the expression of the sulfur quinone oxidoreductase Sqr can improve the rate of oxidizing sulfide into elemental sulfur, and the knockout of the polysulfide transmembrane transporter protein YilD and the sulfur oxidase SoxB contributes to weakening the continuous oxidation of the elemental sulfur into sulfate radicals, so that the yield of the elemental sulfur is improved. Compared with wild bacteria, the engineering bacteria have the advantages that under the condition of the optimal oxidation-reduction potential