Imine reductase mutant and application of imine reductase mutant in synthesis of ivacalcet chiral intermediate

• Main Authors: DU WENTING, LI JUN, ZHU MEICHEN, XU SHENPENG, LIN QINZHE, LYU XUANJIN, YU SISI
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an imine reductase mutant and application of the imine reductase mutant in synthesis of an evanacalcet chiral intermediate, and relates to the technical field of biology. Researches of the inventor find that the imine reductase and the imine reductase mutant provided by the invention can catalyze (R)-1 (1-naphthyl) ethylamine and 3-oxopyrrolidine-1-carboxylic acid tert-butyl ester to synthesize (S)-3-{[(R)-1-(naphthalene-1-yl) ethyl] amino} pyrrolidine-1-carboxylic acid tert-butyl ester, and the conversion rate of catalytic synthesis of the mutant is increased by more than 8 times as high as that of the imine reductase before mutation. The ee value is increased from 63% to 99%. The preparation method of the chiral intermediate (S)-3-{[(R)-1-(naphthalene-1-yl) ethyl] amino} pyrrolidine-1-carboxylic acid tert-butyl ester of evanacalcet, provided by the invention, is simple to operate, mild in reaction condition and green and environment-friendly. 本发明提供了一种亚胺还原酶突变体及其在合成伊万卡塞手性中间体的应用，涉及生物技术领域