Super-resolution three-color fluorescence localization method for high-precision analysis of exosome phenotypes

• Main Authors: CUI YIPING, ZONG SHENFEI, WEI JINXIU, WANG ZHUYUAN
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a super-resolution three-color fluorescence co-localization method for high-precision analysis of exosome phenotypes. According to the method, fluorescence signals generated due to non-specific adsorption can be removed; according to the method, three-color fluorescent labeling, three-color super-resolution imaging and pixel counting of a target exosome are combined, the pixel counting method is realized through MATLAB, and non-specific binding sites can be eliminated on the single-pixel level; in addition, the method also realizes spectrum identification of the exosome membrane protein. 3D phenotype information of various exosomes is obtained by marking three specific proteins; according to the method, different types of exosomes are classified according to protein expression levels; by using the SMLM microscope and the pixel counting method, the detection reliability can be improved to the maximum extent. 本发明公开了一种面向高精度分析外泌体表型的超分辨率三色荧光共定位方法，该方法能够去除由于非特异性吸附产生的荧光信号；该方法结合了目标外泌体的三色荧光标记、三色