Mutant alginate lyase as well as preparation method and application thereof

• Main Authors: WEN HUAMEI, FAN XINJIONG, LIU XIAOLONG
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention is applicable to the technical field of biology, and provides mutant alginate lyase as well as a preparation method and application thereof, the mutant alginate lyase is any one of PspAlgLS270F, PspAlgLG291S and PspAlgLE311P, the mutant alginate lyase is a mutant alginate lyase, and the mutant alginate lyase is a mutant alginate lyase. The amino acid sequences of the PspAlgLS270F, the PspAlgLG291S and the PspAlgLE311P are respectively as shown in SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6 in a sequence table. According to the mutant alginate lyase provided by the invention, alginate lyase which can degrade acetylated alginate in a pseudomonas aeruginosa biological membrane but is poor in stability is taken as a starting enzyme, and a mutant which is greatly improved in stability and can destroy the biological membrane is obtained through mutation; the biofilm can be destroyed when the biofilm is combined with antibiotics, so that the antibiotics can more easily permeate into bacteria to play a