Method for representing ear microcirculation of SD (Sprague Dawley) rat

• Main Authors: ZHANG WEIBO, YAN FEIHONG, SONG HYO-JUNG, JIAN YUQIANG, HAN RONGCHENG
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2024
• Subjects: DIAGNOSIS; HUMAN NECESSITIES; HYGIENE; IDENTIFICATION; MEDICAL OR VETERINARY SCIENCE; SURGERY; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS

The invention relates to the technical field of biology, and discloses a method for representing ear microcirculation of SD (Sprague Dawley) rats, which comprises the following steps: wrapping the SD rats subjected to anesthesia treatment by using a thermal insulation material, and placing the wrapped SD rats on an objective table of a microscope. The ear of the SD rat is fixed on a glass slide below a microscope objective lens. And intravenously injecting a blood vessel marker into the caudal vein of the SD rat. And subcutaneously injecting a lymphatic vessel marker into the ear of the SD rat. And recording the dynamic change process of the blood vessel marker and the lymphatic vessel marker through time sequence imaging so as to characterize the ear microcirculation of the SD rat. By adopting a double-probe co-labeling technology, the ear microcirculation of the SD rat can be more clearly presented, the observation accuracy is high, the height difference of colors can be realized, and the time sequence imag