CRISPR-Cas13 system and application thereof

Relates to a CRISPR-Cas13 (clustered regularly interspaced short palindromic repeats-associated protein 13) system and application thereof, and also relates to Cas13 protein, fusion protein and guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared with SEQ ID NO: 1. The...

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Main Authors LI QIUTING, LIANG JUNBIN, LIANG XINGXIANG, XU HUI, PENG ZHIQIN, SUN YANG, SI KAIWEI, HUANGFU DESHENG
Format Patent
LanguageChinese
English
Published 07.06.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Relates to a CRISPR-Cas13 (clustered regularly interspaced short palindromic repeats-associated protein 13) system and application thereof, and also relates to Cas13 protein, fusion protein and guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared with SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. In some embodiments, the guide polynucleotide comprises an isotropic repeat sequence having at least 70% sequence identity to any of SEQ ID NO: 3, 80-87 and a guide sequence engineered to hybridize to the target RNA. The CRISPR-Cas13 system comprises a Cas13 protein having at least 90% sequence identity compared to SEQ ID NO: 1 or a nucleic acid encoding the Cas13 protein, and a guide polynucleotide or a nucleic acid encoding the guide polynucleotide. 涉及一种CRISPR-Cas13系统及其应用，还涉及一种Cas13蛋白、融合蛋白和指导多核苷酸。所述Cas13蛋白具有与SEQ ID NO：1相比至少90％的序列同一性。所述融合蛋白包含融合至蛋白结构域和/或多肽标签的所述Cas13蛋白。所述指导多核苷酸包含同向重复序列和工程化以与靶RNA杂交的指导序列，所述同向重复序列与SEQ ID NO：
Bibliography:Application Number: CN202380014099