Electrochemical biosensor for lactic acid detection and lactic acid concentration determination method

• Main Authors: GUO YINBIN, ZOU DENGCHAO, CHEN DAJING, HUANG LU, GUO DONG
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The sensor sequentially comprises a substrate layer, an electrode layer, an insulating layer, a reagent layer and a sample layer from bottom to top, and the reagent layer is formed by mixing a buffer system, hydroxyethyl cellulose, a disintegrating agent, a surfactant, an electronic mediator, lactic dehydrogenase and other added stabilizers in proportion. The invention also discloses a lactic acid concentration determination method, which uses the lactic acid detection electrochemical biosensor for determination. According to the electrochemical biosensor for lactic acid detection provided by the invention, lactic dehydrogenase is used as a reaction recognition enzyme to replace common lactic acid oxidase, so that the influence of oxygen partial pressure on a test result is avoided; meanwhile, the electrode layer adopts a multi-electrode structure, and the blood hematocrit and the sample size are detected, so that the error of blood lactic acid detection is reduced, and the accuracy of lactic acid detection i