Construction method and application of saccharomyces cerevisiae for producing itaconic acid

• Main Authors: ZHOU XUAN, CHEN JIAN, PLUGOC CHENG, LIU YANFENG, XU HAO, LI JIANGHUA, LIU LONG, LYU XUEQIN
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a construction method and application of saccharomyces cerevisiae for producing itaconic acid, and belongs to the technical field of fermentation engineering. According to the invention, through metabolic engineering modification and comparison of a promoter for driving CAD (cis-aconitic acid decarboxylase) in acid-resistant saccharomyces cerevisiae, the expression quantity of CAD in cells is improved, then Mtta (mitochondrial tricarboxylic acid transporter) is expressed, a strain with significantly improved itaconic acid yield is obtained, and the accumulation amount in a fermentation supernatant reaches 244.49 mg/L, which is 19.8 times that of a control strain. 本发明公开了一种生产衣康酸的酿酒酵母的构建方法与应用，属于发酵工程技术领域。本发明通过代谢工程改造，在耐酸酿酒酵母中对驱动CAD(顺乌头酸脱羧酶)的启动子进行比较，提高了CAD在胞内的表达量，随后表达了Mtta(线粒体三羧酸转运蛋白)，获得了衣康酸产量显著提高的菌株，其发酵上清液中积累量达244.49mg/L，是对照菌株的19.8倍。