Chimeric Newcastle Disease Virus Expression Of APMV HN And F Proteins

• Main Authors: PALESE PETER, SUN WEINA, GARCIA-SASTRE ADOLFO, KRAMER, FLORIAN, MINA IGNACIO
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

In one aspect, described herein is a recombinant Newcastle disease virus ("NDV") comprising a packaged genome wherein the packaged genome comprises a nucleotide sequence of a Newcastle disease virus genome, wherein a nucleotide sequence encoding an NDV HN protein has been replaced by a nucleotide sequence encoding a variant of the HN protein or of the non-NDV-APMV HN protein of an avian paramyxovirus (APMV) other than NDV, and a nucleotide sequence encoding an NDV F protein has been replaced by a nucleotide sequence encoding a variant of the F protein or of the non-NDV-APMV F protein of an APMV other than NDV. In some embodiments, the packaged genome also comprises a transgene comprising a nucleotide sequence encoding an antigen. Also described herein are compositions comprising such recombinant NDVs and the use of such recombinant NDVs to induce an immune response in a subject. 在一个方面，本文描述了重组新城疫病毒("NDV")，所述重组NDV包含包装的基因组，其中所述包装的基因组包含新城疫病毒基因组的核苷酸序列，其中编码NDV HN蛋白的核苷酸序列已被替换为编码除NDV以外的禽副粘病毒(APMV)的所述HN蛋白或所述非NDV-APMV