Composition and kit for detecting peripheral blood autoimmune antibody AQP4

• Main Authors: LI RUIQIN, HUANG LIULIU, GAO JIEFENG, ZHAO TIANRUN, SHEN JIAWEI, LIU JIYE, XIANG HONGKUN, WU HUIDONG
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of biology, in particular to a composition and a kit for detecting a peripheral blood autoimmune antibody AQP4. According to the present invention, two side arms of AQP4 have antigen binding points, one side is combined with a magnetic bead-coated solid phase AQP4 antigen, and the other side is combined with subsequently added acridinium esterified AQP4 expressing 293T cells so as to form a bridge structure, and finally acridinium ester and a substrate thereof are subjected to chromogenic reaction detection. A fluorescence immune cell staining method and a chemiluminescence method are combined, a 293T cell expressing AQP4 is used as an antigen, acridinium ester is directly marked on the cell, the cell plays a role in amplifying a signal, and the detection sensitivity is improved. The antigen is directly expressed on cells, has the highest activity, and can improve the sensitivity and specificity of detection. While high detection sensitivity is guaranteed, time and