High-flux micro-fluidic chip for nucleic acid detection

• Main Authors: LI RUIQIN, GAO JIEFENG, SHEN JIAWEI, XIANG HONGKUN, WU HUIDONG, ZHANG JING
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2024
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention relates to a high-flux micro-fluidic chip for nucleic acid detection. The high-flux micro-fluidic chip comprises a sample injection hole (1), a flow channel (2), a reaction cavity (3) and an exhaust hole (4), the flow channel (2) is communicated with the sample injection hole (1) and the reaction cavity (3); the exhaust hole (4) is positioned at the tail end of the runner (2); the reaction cavity (3) is divided into two chambers, and the pre-embedded substance A (301) and the pre-embedded substance B (302) are pre-embedded in the two chambers of the reaction cavity (3) respectively; the pre-embedded substance A (301) and the pre-embedded substance B (302) are respectively a nucleic acid detection reaction reagent and a substance for air-drying polymerase chain reaction. According to the invention, the sample can be placed in the reaction cavity without additional steps such as centrifugation, oil sealing and the like, the flux can be randomly switched from 1 to 32, and the kit can be used for no