Soybean cyst nematode resistance related M1979SNP molecular marker, primer and detection method and application thereof

• Main Authors: MA JUNKUI, CHEN YAN, ZHANG HAIPING, MU ZHIXIN
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of molecular biology, and provides a soybean cyst nematode resistance related M1979SNP molecular marker, a primer and a detection method and application thereof, and the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1 or SEQ ID NO: 2; the molecular marker is located at the 1641679th site of the 18th chromosome of the soybean. The marker designed based on the KASP reaction principle and the single base difference of the material can be used for detecting the soybean material at high throughput, the KASP marker M1979 is adopted, and the experiment proves that the SNP marker developed by the invention can be used for detecting the resistance of the soybean cyst nematode No.4 race on the breeding material in the early stage of the variety by detecting the genotype of the SNP marker, and the SNP marker can be used for detecting the resistance of the soybean cyst nematode No.4 race in the early stage of the variety. The selection efficiency of dis