Application of phosphokinase KinA in improving yield of bacillus active secondary metabolites

• Main Authors: LIU ZEHUA, TIAN YONGQING, HU XIN, XIAO YATING, XU HANHONG, JIA JIPENG
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an application of phosphokinase KinA in improving the yield and/or bacteriostatic activity of active secondary metabolites of bacillus. The active secondary metabolites are iturin family compounds and polyketone compounds. Research finds that the phosphokinase has a regulation effect in yield improvement of iturin family compounds and polyketone compounds, the yield of active compounds of bacillus strains of overexpressed KinA genes is increased, the yield of secondary metabolite bacillomycin D is increased by 1.58 times, the yield of oxydidifficidin is increased by 1.78 times, the antibacterial activity on botrytis cinerea is increased by 38%, and the activity of the phosphokinase on the botrytis cinerea is increased by 38%. The bacteriostatic activity on magnaporthe oryzae is improved by 25%, and the application value of the bacillus as a green biopesticide is improved. 本发明公开了磷酸激酶KinA在提高芽孢杆菌活性次生代谢产物产量和/或抑菌活性中的应用，所述活性次生代谢产物为伊枯草素家族化合物和聚酮类化合物。本发明研究发现磷酸激酶在伊枯草素家族化合物和聚酮类化合物的产量提高中的调控作用，过表达K