Protein glutaminase mutant and application thereof

• Main Authors: FANG HAO, WANG YONGHUA, LAN DONGMING, LONG YATING
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a protein glutaminase mutant and an application thereof. According to the mutant FPG-R205K, single-point mutation is carried out on the 205th site of the protein glutaminase of Flavobacterium sp.316 derived from the Porosea Gymph, and the enzyme activity of the obtained mutant FPG-R205K is improved by 6.37 times compared with that of a wild type, so that the industrial production requirements can be better met. The FPG mutant enriches the variety of PG, and provides reference and guidance for industrial application research of the FPG mutant. 本发明公开了一种蛋白质谷氨酰胺酶突变体及其应用。在本发明中通过对来源于波罗的海格但斯克湾的黄杆菌属(Flavobacterium sp.316)蛋白质谷氨酰胺酶的第205位进行单点突变，所得突变体FPG-R205K的酶活力相比野生型提高6.37倍，能够更好地满足工业生产需求。本发明的FPG突变体丰富了PG的种类，并为其工业应用研究提供借鉴和指导。