Temperature-selectable FRET cartridge signal conduction

• Main Authors: SHAH, AMIT, H, GUILD AMEYA SHANKAR, GIGLEY, MICHAEL, J
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

A multiplexed nucleic acid amplification and detection system that can be used to detect the presence of a plurality of specific nucleic acid sequences or single nucleotide polymorphisms (i.e., "SNPs") in a temperature dependent manner using only a single fluorescence detection channel of a nucleic acid analyzer. The techniques may be performed using a standard PCR instrument equipped for fluorescence detection or monitoring. 一种多重化核酸扩增和检测系统，所述多重化核酸扩增和检测系统可用于仅使用核酸分析仪的单个荧光检测通道以温度依赖性方式检测多个特定核酸序列或单核苷酸多态性(即，"SNP")的存在。可以使用配备用于荧光检测或监测的标准PCR仪器进行所述技术。