Enzyme-labeled antibody diluent for ELISA (enzyme-linked immunosorbent assay) detection kit and preparation method of enzyme-labeled antibody diluent

• Main Authors: XING JIAPENG, HUANG DANDAN, WU XINGLIANG, WANG YALING, LI PUJUN, XIAO WENHUI, LI YONGHAN, JIA YUQI, ZHU WENPENG, WEN JINGLIANG, JIN LING, YANG XUN, SHANG LILI
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to an enzyme-labeled antibody diluent for an ELISA (enzyme-linked immunosorbent assay) detection kit and a preparation method of the enzyme-labeled antibody diluent, belonging to the technical field of enzyme-linked immunosorbent assay. The enzyme-labeled antibody diluent is prepared from the following components in percentage by mass: 0.1 to 1.0 percent of bovine serum albumin, 0.1 to 1.0 percent of polyvinylpyrrolidone, 1 to 8 mM of FeSO4, 0.02 to 0.2 percent of thioredoxin and 0.01 to 0.1 percent of preservative. The components in the enzyme-labeled antibody diluent provided by the invention cooperate with each other, so that the stability of the enzyme-labeled antibody is remarkably improved, and the ELISA detection kit containing the enzyme-labeled antibody diluent has better accuracy and stability. Meanwhile, the enzyme-labeled antibody diluent can dilute the enzyme-labeled antibody to a working concentration before the product leaves the factory, and the enzyme-labeled antibody di