VEGF magnetic particle chemiluminescence detection reagent, kit, detection method and application

• Main Authors: SUN CHAN, ZUO YUNGUO, ZHANG NIANLIN, ZHONG YUQIN, LIU CHANG, MA HONGCHAO, DENG WEIRONG
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of chemiluminescence in-vitro diagnosis, and discloses a VEGF magnetic particle chemiluminescence detection reagent, a kit, a detection method and application, the VEGF magnetic particle chemiluminescence detection reagent comprises a VEGF antibody-1 coated Tosyl magnetic bead, an acridinium ester labeled VEGF antibody-2, a calibrator and a quality control material.By means of the VEGF magnetic particle chemiluminescence detection reagent, interference of biotin of a sample on a detected value is avoided, and the detection accuracy is improved. Meanwhile, as the VEGF antibody-1 is treated at 37 DEG C when coated with the Tosyl magnetic beads, the stability of the antibody is greatly improved, the 0 value is low, the repeatability and the linear relation are better, and the sample coincidence rate is better. 本发明涉及化学发光体外诊断技术领域，公开了一种VEGF磁微粒化学发光检测试剂、试剂盒、检测方法和应用，包括VEGF抗体-1包被Tosyl磁珠、吖啶酯标记VEGF抗体-2、校准品、质控品，通过本发明的VEGF磁微粒化学发光检测试剂避免了样本的生物素对测值的干扰，同时由于VEGF抗体-1包被Tosyl磁珠时在37℃进行处理