Reagent and method for detecting chlamydia psittaci based on IncA protein or IncA gene of chlamydia psittaci

• Main Authors: SONG LIHUA, AN XIAOPING, MA YUEZHEN, CHE FUYOU, TONG YIGANG
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a reagent and a method for detecting chlamydia psittaci based on an IncA protein or an IncA gene of the chlamydia psittaci. The specific primer probe group provided by the invention is composed of a primer Cps977F as shown in SEQ ID NO: 3, a primer Cps1105R as shown in SEQ ID NO: 4 and a probe Cps1010-P as shown in SEQ ID NO: 5. The DSBC-IncA fusion protein provided by the invention comprises a DSBC segment as shown in the 1st to 236th sites in SEQ ID NO: 2 and an IncA segment as shown in the 298th to 639th sites in the SEQ ID NO: 2. According to the specific primer probe group and the corresponding fluorescent PCR method provided by the invention, the DSBC-IncA fusion protein provided by the invention and the corresponding serological diagnosis method provided by the invention, large-scale rapid screening and accurate detection of the chlamydia psittaci can be realized. 本发明公开了基于鹦鹉热衣原体的IncA蛋白或IncA基因检测鹦鹉热衣原体的试剂和方法。本发明提供的特异引物探针组由SEQ ID NO：3所示引物Cps977F、SEQ ID NO：4所示引物Cps1105R和SEQ ID NO：5所