Klebsiella engineering bacterium constructed on basis of constitutive promoter and application of Klebsiella engineering bacterium

• Main Authors: BAO MENGYUAN, ZHOU XIANDA, FU YANAN, QIU LEQUAN, WU SHIJIN, LI TONGTONG
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS; CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING ORPROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FIRE-FIGHTING; HUMAN NECESSITIES; LIFE-SAVING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a Klebsiella engineering bacterium constructed based on a constitutive promoter and application, the engineering bacterium is constructed by using a PAEs esterase gene pehA as an exogenous gene, connecting the exogenous gene between BamHI and Hind III restriction enzyme cutting sites of a pET28a (+) expression vector of which a lactose operon lacI and a promoter sequence thereof are knocked out and the constitutive promoter is used for replacing a T7 promoter to construct a recombinant expression vector, and constructing the Klebsiella engineering bacterium based on the constitutive promoter. And transforming Klebsiella variicola to obtain the strain. According to the constitutive expression vector pET-PJ23105 * disclosed by the invention, klebsiella can be used as a host, and efficient and constant expression of an exogenous target gene is realized; the DEP and DBP degradation rates of the recombinant strain are enhanced to 92% and 97%, and the recombinant strain can be applied to deg