Method for rapidly detecting ofloxacin by using ratio type fluorescent probe

• Main Authors: LI TAIHUA, DAI JING, DUAN MINGKUN, JIANG WENPING, LI WEI, YANG JIN, ZHANG KUN
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for rapidly detecting ofloxacin by using a ratio type fluorescent probe, and belongs to the technical field of antibiotic detection. The fluorescence intensity of a to-be-detected solution added with a fluorescence probe GSH-CuNCs is measured by adopting a fluorescence spectrometer, and compared with the fluorescence intensity of GSH-CuNCs, if the intensity of green fluorescence emitted at 510 nm is enhanced, the to-be-detected solution contains an ofloxacin component. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, rapidness, high sensitivity, good selectivity, low detection limit, low cost and the like, does not need large-scale instruments and equipment and professional operators, has good standard addition recovery rate in actual water sample detection, is reliable in detection result and has good practicability. 本发明公开了一种比率型荧光探针快速检测氧氟沙星的方法，属于抗生素检测技术领域。本发明采用荧光光谱仪测定加有荧光探针GSH-CuNCs的待测溶液的荧光强度，与GSH-CuNCs的荧光强度相比，如在51