Recombinant elastin, preparation method and application thereof

• Main Authors: XU MIAO, ZHANG CHUANPENG, QIANG BANGMING, LIU MINGLU, JIN HAIFENG, LI SHUHONG
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a recombinant elastin and a preparation method and application thereof, and belongs to the technical field of binding tissue peptides, the recombinant elastin is formed by sequentially connecting the following modules in series: a signal peptide, a truncated elastin, a His tag, a connexon and an elastin exon 30; the nucleotide sequence of the truncated elastin is shown as any one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 and SEQ ID NO. 5 in a sequence table. After the nucleotide sequence of the truncated elastin is optimized, the constructed recombinant expression vector has higher capability of expressing the recombinant elastin; the recombinant elastin with the optimized nucleotide sequence has the advantages that the effects of promoting fibroblast proliferation and promoting fibroblast to secrete procollagen are obviously better than those of the recombinant elastin before the nucleotide sequence is optimized. 本发明提供一种重组弹性蛋白、制备方法及其应用，属于结合组织肽技术领域，所述重组弹性蛋白由以下模块依次串联：信号肽、