Bacillus subtilis and application thereof in preparation of energy milk tea tablets rich in alkaloids

• Main Authors: ZHUO SONGSHAN, GUO PEIXIN, DENG MAOCHENG, SHEN XIAOYA, LI MEITING, LI JING, DU CHUYAO, CAI SHIPING, LUO TIANLIN, WANG YAO, CHEN BAOYI, LIU YUSHAN, SITU MANQUAN, CHEN HANCHUN, LU LEYI, LUO QIURU, ZHOU WENJUN, LONG YI
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COFFEE; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TEA; THEIR SUBSTITUTES; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention discloses bacillus subtilis and application thereof in preparation of energy milk tea tablets rich in alkaloid. The name of the bacillus subtilis is bacillus subtilis FR005, the preservation number of the bacillus subtilis FR005 is GDMCC (China General Microbiological Culture Collection Center) NO: 63456, and the bacillus subtilis FR005 is preserved in Guangdong Province on May 11, 2023. The preparation method comprises the following steps: firstly fermenting a mixed solution of yerba mate powder and water by adopting bacillus subtilis, and then carrying out reflux extraction and centrifugal separation to remove residues, so as to obtain an extract; and mixing the extract, macadamia nuts, full-fat sugar-free milk powder, cane sugar, maltodextrin, yerba mate powder and taurine, grinding, boiling into paste, molding by casting, drying and the like to obtain the energy milk tea tablets rich in alkaloid. Compared with an energy milk tea tablet which is not subjected to a fermentation process, the en