Method for detecting isomer of aspartic acid

• Main Authors: LIU SHUYUAN, LI CONGBO, LI SUXIA, LI HAISONG, WU CUNJING, MA JING, YAN LING, LI BINSHUI
• Format: Patent
• Language: Chinese; English
• Published: 14.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an aspartic acid isomer detection method, and belongs to the technical field of isomer detection. The method comprises the following steps: (1) preparing a reference solution containing L-aspartic acid and D-aspartic acid; (2) detecting the reference substance solution in the step (1) by using a high performance liquid chromatography, and drawing a standard curve of the L-aspartic acid and D-aspartic acid reference substance according to the obtained peak area to obtain a concentration-peak area linear regression equation of the L-aspartic acid and D-aspartic acid reference substance; and (3) taking a to-be-detected sample, detecting the to-be-detected sample by using the same conditions in the step (2), substituting the obtained peak area into the concentration-peak area linear regression equation, and calculating the content of aspartic acid in the to-be-detected sample. The method is high in detection sensitivity and low in detection limit, the aspartic acid isomer is accurately and