Swine streptococcosis indirect ELISA antibody detection kit and detection method

• Main Authors: ZHU LIANGQUAN, LIU YAN, WANG XIULI, XIN LINGXIANG, ZHANG YIZHI, HU YUNHAO, WANG QI, TONG RENDONG, WANG HAOJIE, ZHANG CUNSHUAI, YAO WENSHENG, JIN MEIJUN
• Format: Patent
• Language: Chinese; English
• Published: 14.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a swine streptococcosis indirect ELISA antibody detection kit and a detection method, and belongs to the technical field of biological detection. The kit comprises a coating plate, a sample diluent, an enzyme conjugate, a positive control, a negative control, a color developing solution A, a color developing solution B, a concentrated washing solution, a stop solution, a sample adding groove, a sealing plate and a serum diluting plate. According to the invention, a prokaryotic expression system is used for expressing a streptococcus suis Sao-MRP-EF fusion protein, and the purified Sao-MRP-EF fusion protein is used as a coating antigen to establish a streptococcus suis indirect ELISA antibody detection method. The prepared streptococcus suis Sao-MRP-EF fusion egg is good in antigenicity, an indirect ELISA antibody detection method is established on the basis of the streptococcus suis Sao-MRP-EF fusion egg, and the streptococcus suis Sao-MRP-EF fusion egg has the advantages of being high