Device and method suitable for detecting and processing free amino acid in meat sample

• Main Authors: DENG JING, QIAO MINGFENG, YI YUWEN, FAN WENJIAO, WANG YONGJIANG, PENG YIQIN, CAI XUEMEI
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of meat product detection, in particular to a treatment device and method suitable for detecting free amino acid in a meat sample, and the method comprises the following steps: repeatedly mincing the meat sample for three times by using a meat mincer, and uniformly mixing to obtain a pretreated sample; adding a proper amount of trichloroacetic acid into the pretreated sample, carrying out ultrasonic treatment at 30-40 DEG C for 30-50 minutes, and extracting free amino acid to obtain a first sample; then centrifuging the first sample at 4 DEG C at 10000 r/min for 10 min, taking the supernatant, and filtering the supernatant with a 0.22 [mu] m pinhole filter membrane to obtain a sample to be detected; the method comprises the following steps: detecting a to-be-detected sample by using an automatic amino acid analyzer, heating at 130 DEG C, detecting a derivative product by using a visible spectrophotometric detector, determining the nature of retention time, and quan